After awakening from a four-year coma, a former assassin wreaks vengeance on the team of assassins who betrayed her.
Characters and their actors
- Lucy Liu as O-Ren Ishii (code name Cottonmouth), a former Deadly Viper who has become the leader of the Japanese yakuza.
- Julie Dreyfus as Sofie Fatale, O-Ren's lawyer, confidante, and second lieutenant. She is also a former protégée of Bill's and is present at the wedding chapel massacre.
- Sonny Chiba as Hattori Hanzō, a sushi chef and long-retired master swordsmith.
- Chiaki Kuriyama as Gogo Yubari, O-Ren's Japanese schoolgirl bodyguard.
- Jun Kunimura as Boss Tanaka, a yakuza whom O-Ren executes after he ridicules her ethnicity and gender.
- Kenji Ohba as Shiro, Hattori Hanzo's employee.
- Kazuki Kitamura as Boss Koji, a yakuza working for O-Ren. He also appeared as Bodyguard #2 in O-Ren's army, the Crazy 88.
- Yuki Kazamatsuri as the Proprietress of the House of Blue Leaves.
- Sakichi Sato as "Charlie Brown", a House of Blue Leaves employee who wears a kimono similar to the shirt worn by the Peanuts character.
- The 5.6.7.8's (Sachiko Fuji, Yoshiko Yamaguchi and Ronnie Yoshiko Fujiyama) as themselves, performing at the House of Blue Leaves.
- Yōji Tanaka as Crazy 88 #3
- Issey Takahashi as Crazy 88 #4
- Juri Manase as Crazy 88 #6
- Akaji Maro as Boss Ozawah
- Ai Maeda as O-Ren (anime sequence) (voice)
- Naomi Kusumi as Boss Matsumoto (anime sequence) (voice)
- Hikaru Midorikawa as Pretty Riki (anime sequence) (voice)